bogdan's blog

Zip Nucleic Acids: new high affinity oligonucleotides as potent primers for PCR and reverse transcription

Zip Nucleic Acids: new high affinity oligonucleotides as potent primers for PCR and reverse transcription - Most nucleic acid-based technologies rely upon sequence recognition between an oligonucleotide and its nucleic acid target.

Expression-independent gene trap vectors for random and targeted mutagenesis in embryonic stem cells

Expression-independent gene trap vectors for random and targeted mutagenesis in embryonic stem cells - Promoterless gene trap vectors have been widely used for high-efficiency gene targeting and random mutagenesis in embryonic stem (ES) cells.

A real-time fluorescence method for enzymatic characterization of specialized human DNA polymerases

A real-time fluorescence method for enzymatic characterization of specialized human DNA polymerases - Specialized DNA polymerases are involved in DNA synthesis during base-excision repair and translesion synthesis across a wide range of chemically modified DNA templates.

RNA polymerase III can drive polycistronic expression of functional interfering RNAs designed to resemble microRNAs

RNA polymerase III can drive polycistronic expression of functional interfering RNAs designed to resemble microRNAs - In both research and therapeutic applications of RNA interference, it is often advantageous to silence several targets simultaneously.

Analysis of splicing patterns by pyrosequencing

Analysis of splicing patterns by pyrosequencing - Several different mRNAs can be produced from a given pre-mRNA by regulated alternative splicing, or as the result of deregulations that may lead to pathological states.