Stem Cells

An Effective Approach to Prevent Immune Rejection of Human ESC-Derived Allografts

Stem Cell: most read - чт, 2014-01-02 02:00
Zhili Rong, Meiyan Wang, Zheng Hu, Martin Stradner, Shengyun Zhu, Huijuan Kong, Huanfa Yi, Ananda Goldrath, Yong-Guang Yang, Yang Xu, Xuemei Fu. Human embryonic stem cells (hESCs) hold great promise for cell therapy as a source of diverse differentiated cell types. One key bottleneck to realizing such potential is allogenic immune rejectio....
Категории: Biology, Journals, Stem Cells

Human Pluripotent Stem Cell Culture: Considerations for Maintenance, Expansion, and Therapeutics

Stem Cell: most read - чт, 2014-01-02 02:00
Kevin G. Chen, Barbara S. Mallon, Ronald D.G. McKay, Pamela G. Robey. Human pluripotent stem cells (hPSCs) provide powerful resources for application in regenerative medicine and pharmaceutical development. In the past decade, various methods have been developed for....
Категории: Biology, Journals, Stem Cells

In Vivo Direct Reprogramming of Reactive Glial Cells into Functional Neurons after Brain Injury and in an Alzheimer’s Disease Model

Stem Cell: most read - чт, 2013-12-19 02:00
Ziyuan Guo, Lei Zhang, Zheng Wu, Yuchen Chen, Fan Wang, Gong Chen. Loss of neurons after brain injury and in neurodegenerative disease is often accompanied by reactive gliosis and scarring, which are difficult to reverse with existing treatment approaches. Here, ....
Категории: Biology, Journals, Stem Cells

Genetic Modification and Screening in Rat Using Haploid Embryonic Stem Cells

Stem Cell: most read - чт, 2013-12-19 02:00
Wei Li, Xin Li, Tianda Li, Ming-Gui Jiang, Haifeng Wan, Guan-Zheng Luo, Chunjing Feng, Xiaolong Cui, Fei Teng, Yan Yuan, Quan Zhou, Qi Gu, Ling Shuai, Jiahao Sha, Yamei Xiao, Liu Wang, Zhonghua Liu, Xiu-Jie Wang, Xiao-Yang Zhao, Qi Zhou. The rat is an important animal model in biomedical research, but practical limitations to genetic manipulation have restricted the application of genetic analysis. Here we report the derivation of....
Категории: Biology, Journals, Stem Cells

Lgr5+ Stem Cells Are Indispensable for Radiation-Induced Intestinal Regeneration

Stem Cell: most read - чт, 2013-12-12 02:00
Ciara Metcalfe, Noelyn M. Kljavin, Ryan Ybarra, Frederic J. de Sauvage. The intestinal epithelium continually self-renews and can rapidly regenerate after damage. Lgr5 marks mitotically active intestinal stem cells (ISCs). Importantly, intestinal homeostasis ca....
Категории: Biology, Journals, Stem Cells

Redefining the In Vivo Origin of Metanephric Nephron Progenitors Enables Generation of Complex Kidney Structures from Pluripotent Stem Cells

Stem Cell: most read - чт, 2013-12-12 02:00
Atsuhiro Taguchi, Yusuke Kaku, Tomoko Ohmori, Sazia Sharmin, Minetaro Ogawa, Hiroshi Sasaki, Ryuichi Nishinakamura. Recapitulating three-dimensional (3D) structures of complex organs, such as the kidney, from pluripotent stem cells (PSCs) is a major challenge. Here, we define the developmental origins of the me....
Категории: Biology, Journals, Stem Cells

Functional Repair of CFTR by CRISPR/Cas9 in Intestinal Stem Cell Organoids of Cystic Fibrosis Patients

Stem Cell: most read - чт, 2013-12-05 02:00
Gerald Schwank, Bon-Kyoung Koo, Valentina Sasselli, Johanna F. Dekkers, Inha Heo, Turan Demircan, Nobuo Sasaki, Sander Boymans, Edwin Cuppen, Cornelis K. van der Ent, Edward E.S. Nieuwenhuis, Jeffrey M. Beekman, Hans Clevers. Single murine and human intestinal stem cells can be expanded in culture over long time periods as genetically and phenotypically stable epithelial organoids. Increased cAMP levels induce rapid sw....
Категории: Biology, Journals, Stem Cells

Correction of a Genetic Disease in Mouse via Use of CRISPR-Cas9

Stem Cell: most read - чт, 2013-12-05 02:00
Yuxuan Wu, Dan Liang, Yinghua Wang, Meizhu Bai, Wei Tang, Shiming Bao, Zhiqiang Yan, Dangsheng Li, Jinsong Li. The CRISPR-Cas9 system has been employed to generate mutant alleles in a range of different organisms. However, so far there have not been reports of use of this system for efficient correction of....
Категории: Biology, Journals, Stem Cells

Induction of a Human Pluripotent State with Distinct Regulatory Circuitry that Resembles Preimplantation Epiblast

Stem Cell: most read - чт, 2013-12-05 02:00
Yun-Shen Chan, Jonathan Göke, Jia-Hui Ng, Xinyi Lu, Kevin Andrew Uy Gonzales, Cheng-Peow Tan, Wei-Quan Tng, Zhong-Zhi Hong, Yee-Siang Lim, Huck-Hui Ng. Human embryonic stem cells (hESCs) are derived from the inner cell mass of the blastocyst. Despite sharing the common property of pluripotency, hESCs are notably distinct from epiblast cells of th....
Категории: Biology, Journals, Stem Cells

Human iPSC-Based Modeling of Late-Onset Disease via Progerin-Induced Aging

Stem Cell: most read - чт, 2013-12-05 02:00
Justine D. Miller, Yosif M. Ganat, Sarah Kishinevsky, Robert L. Bowman, Becky Liu, Edmund Y. Tu, Pankaj K. Mandal, Elsa Vera, Jae-won Shim, Sonja Kriks, Tony Taldone, Noemi Fusaki, Mark J. Tomishima, Dimitri Krainc, Teresa A. Milner, Derrick J. Rossi, Lorenz Studer. Reprogramming somatic cells to induced pluripotent stem cells (iPSCs) resets their identity back to an embryonic age and, thus, presents a significant hurdle for modeling late-onset disorders. In ....
Категории: Biology, Journals, Stem Cells

Distinct Functions for Wnt/β-Catenin in Hair Follicle Stem Cell Proliferation and Survival and Interfollicular Epidermal Homeostasis

Stem Cell: most read - чт, 2013-12-05 02:00
Yeon Sook Choi, Yuhang Zhang, Mingang Xu, Yongguang Yang, Mayumi Ito, Tien Peng, Zheng Cui, Andras Nagy, Anna-Katerina Hadjantonakis, Richard A. Lang, George Cotsarelis, Thomas Andl, Edward E. Morrisey, Sarah E. Millar. Wnt/β-catenin signaling is a central regulator of adult stem cells. Variable sensitivity of Wnt reporter transgenes, β-catenin’s dual roles in adhesion and signaling, and hair follicle degradation....
Категории: Biology, Journals, Stem Cells

Enhanced Telomere Rejuvenation in Pluripotent Cells Reprogrammed via Nuclear Transfer Relative to Induced Pluripotent Stem Cells

Stem Cell: most read - чт, 2013-11-21 02:00
Rongrong Le, Zhaohui Kou, Yonghua Jiang, Ming Li, Bo Huang, Wenqiang Liu, Hui Li, Xiaochen Kou, Wanzhong He, Karl Lenhard Rudolph, Zhenyu Ju, Shaorong Gao. Although somatic cell nuclear transfer (SCNT) and induction of pluripotency (to form iPSCs) are both recognized reprogramming methods, there has been relatively little comparative analysis of the ....
Категории: Biology, Journals, Stem Cells

Contractile Forces Sustain and Polarize Hematopoiesis from Stem and Progenitor Cells

Stem Cell: most read - чт, 2013-11-21 02:00
Jae-Won Shin, Amnon Buxboim, Kyle R. Spinler, Joe Swift, David A. Christian, Christopher A. Hunter, Catherine Léon, Christian Gachet, P.C. Dave P. Dingal, Irena L. Ivanovska, Florian Rehfeldt, Joel Anne Chasis, Dennis E. Discher. Self-renewal and differentiation of stem cells depend on asymmetric division and polarized motility processes that in other cell types are modulated by nonmuscle myosin-II (MII) forces and matrix ....
Категории: Biology, Journals, Stem Cells

Fundamental Differences in Dedifferentiation and Stem Cell Recruitment during Skeletal Muscle Regeneration in Two Salamander Species

Stem Cell: most read - чт, 2013-11-21 02:00
Tatiana Sandoval-Guzmán, Heng Wang, Shahryar Khattak, Maritta Schuez, Kathleen Roensch, Eugeniu Nacu, Akira Tazaki, Alberto Joven, Elly M. Tanaka, András Simon. Salamanders regenerate appendages via a progenitor pool called the blastema. The cellular mechanisms underlying regeneration of muscle have been much debated but have remained unclear. Here we app....
Категории: Biology, Journals, Stem Cells

The let-7/LIN-41 Pathway Regulates Reprogramming to Human Induced Pluripotent Stem Cells by Controlling Expression of Prodifferentiation Genes

Stem Cell: most read - чт, 2013-11-14 02:00
Kathleen A. Worringer, Tim A. Rand, Yohei Hayashi, Salma Sami, Kazutoshi Takahashi, Koji Tanabe, Megumi Narita, Deepak Srivastava, Shinya Yamanaka. Reprogramming differentiated cells into induced pluripotent stem cells (iPSCs) promotes a broad array of cellular changes. Here we show that the let-7 family of microRNAs acts as an inhibit....
Категории: Biology, Journals, Stem Cells

Polycomb Repressive Complex 2 Regulates Normal Hematopoietic Stem Cell Function in a Developmental-Stage-Specific Manner

Stem Cell: most read - чт, 2013-11-14 02:00
Huafeng Xie, Jian Xu, Jessie H. Hsu, Minh Nguyen, Yuko Fujiwara, Cong Peng, Stuart H. Orkin. Recent studies point to a pivotal role of Polycomb repressive complex 2 (PRC2) in stem cell function and cancer. Loss-of-function approaches targeting individual PRC2 subunits have, however, gener....
Категории: Biology, Journals, Stem Cells

Transient Activation of Autophagy via Sox2-Mediated Suppression of mTOR Is an Important Early Step in Reprogramming to Pluripotency

Stem Cell: most read - чт, 2013-11-07 02:00
Shuo Wang, Pengyan Xia, Buqing Ye, Guanling Huang, Jing Liu, Zusen Fan. Autophagy is an essential cellular mechanism that degrades cytoplasmic proteins and organelles to recycle their components. Here we show that autophagy is required for reprogramming of somatic cel....
Категории: Biology, Journals, Stem Cells

Transient Maternal IL-6 Mediates Long-Lasting Changes in Neural Stem Cell Pools by Deregulating an Endogenous Self-Renewal Pathway

Stem Cell: most read - чт, 2013-11-07 02:00
Denis Gallagher, Andreea A. Norman, Cameron L. Woodard, Guang Yang, Andrée Gauthier-Fisher, Masashi Fujitani, John P. Vessey, Gonzalo I. Cancino, Nadia Sachewsky, Knut Woltjen, Michael P. Fatt, Cindi M. Morshead, David R. Kaplan, Freda D. Miller. The mechanisms that regulate the establishment of adult stem cell pools during normal and perturbed mammalian development are still largely unknown. Here, we asked whether a maternal cytokine surg....
Категории: Biology, Journals, Stem Cells

Deciphering Hematopoietic Stem Cells in Their Niches: A Critical Appraisal of Genetic Models, Lineage Tracing, and Imaging Strategies

Stem Cell: most read - чт, 2013-11-07 02:00
Chacko Joseph, Julie M. Quach, Carl R. Walkley, Steven W. Lane, Cristina Lo Celso, Louise E. Purton. In recent years, technical developments in mouse genetics and imaging equipment have substantially advanced our understanding of hematopoietic stem cells (HSCs) and their niche. The availability o....
Категории: Biology, Journals, Stem Cells