Feed aggregator

The DNA Translocase FANCM/MHF Promotes Replication Traverse of DNA Interstrand Crosslinks

Molecular Cell (most read) - Thu, 2013-10-24 02:00
Jing Huang, Shuo Liu, Marina A. Bellani, Arun Kalliat Thazhathveetil, Chen Ling, Johan P. de Winter, Yinsheng Wang, Weidong Wang, Michael M. Seidman. The replicative machinery encounters many impediments, some of which can be overcome by lesion bypass or replication restart pathways, leaving repair for a later time. However, interstrand crossli....

Braking BRAF: AMPK Leaves ERK Stranded in the Desert

Molecular Cell (most read) - Thu, 2013-10-24 02:00
Matthew Martin, Richard Marais. In this issue, Shen et al. (2013) show that BRAF phosphorylation by AMPK attenuates BRAF signaling and inhibits cell proliferation. This identifies BRAF as a novel AMPK substrate and further revea....

Design, Synthesis, and Biological Evaluation of an Allosteric Inhibitor of HSET that Targets Cancer Cells with Supernumerary Centrosomes

Chemistry and Biology (most read) - Thu, 2013-10-24 02:00
Ciorsdaidh A. Watts, Frances M. Richards, Andreas Bender, Peter J. Bond, Oliver Korb, Oliver Kern, Michelle Riddick, Paul Owen, Rebecca M. Myers, Jordan Raff, Fanni Gergely, Duncan I. Jodrell, Steven V. Ley. Centrosomes associate with spindle poles; thus, the presence of two centrosomes promotes bipolar spindle assembly in normal cells. Cancer cells often contain supernumerary centrosomes, and to avoi....
Categories: Biology, Chemistry, Journals

Autophosphorylation Activity of a Soluble Hexameric Histidine Kinase Correlates with the Shift in Protein Conformational Equilibrium

Chemistry and Biology (most read) - Thu, 2013-10-24 02:00
Marta Wojnowska, Jun Yan, Ganesh N. Sivalingam, Adam Cryar, Jayesh Gor, Konstantinos Thalassinos, Snezana Djordjevic. In a commonly accepted model, in response to stimuli, bacterial histidine kinases undergo a conformational transition between an active and inactive form. Structural information on histidine kinas....
Categories: Biology, Chemistry, Journals

Biochemical and Structural Studies of Conserved Maf Proteins Revealed Nucleotide Pyrophosphatases with a Preference for Modified Nucleotides

Chemistry and Biology (most read) - Thu, 2013-10-24 02:00
Anatoli Tchigvintsev, Dmitri Tchigvintsev, Robert Flick, Ana Popovic, Aiping Dong, Xiaohui Xu, Greg Brown, Wenyun Lu, Hong Wu, Hong Cui, Ludmila Dombrowski, Jeong Chan Joo, Natalia Beloglazova, Jinrong Min, Alexei Savchenko, Amy A. Caudy, Joshua D. Rabinowitz, Alexey G. Murzin, Alexander F. Yakunin. Maf (for multicopy associated filamentation) proteins represent a large family of conserved proteins implicated in cell division arrest but whose biochemical activity remains unknown. Here, we sho....
Categories: Biology, Chemistry, Journals

Site-Specific Differences in Proteasome-Dependent Degradation of Monoubiquitinated α-Synuclein

Chemistry and Biology (most read) - Thu, 2013-10-24 02:00
Tharindumala Abeywardana, Yu Hsuan Lin, Ruth Rott, Simone Engelender, Matthew R. Pratt. The formation of toxic aggregates composed largely of the protein α-synuclein are a hallmark of Parkinson’s disease. Evidence from both early-onset forms of the disease in humans and animal models....
Categories: Biology, Chemistry, Journals

Curing Baldness Activates Antibiotic Production

Chemistry and Biology (most read) - Thu, 2013-10-24 02:00
Keith F. Chater. Kalan and colleagues describe an unusual way of activating a “silent” gene cluster for the biosynthesis of a new antibiotic by analyzing and curing the aerial growth defect of an old Streptomyc....
Categories: Biology, Chemistry, Journals

A Jack of All Trades to Combat Drug-Resistant Gram-Negative Bacteria

Chemistry and Biology (most read) - Thu, 2013-10-24 02:00
Thomas Kodadek. In this issue of Chemistry & Biology, Jahnsen and colleagues describe a multimodal mechanism of action of an unusual peptide-peptoid hybrid with broad-spectrum antibacterial activity. T....
Categories: Biology, Chemistry, Journals

Precocious Acquisition of Neuroepithelial Character in the Eye Field Underlies the Onset of Eye Morphogenesis

Developmental Cell (most read) - Thu, 2013-10-24 02:00
Kenzo Ivanovitch, Florencia Cavodeassi, Stephen W. Wilson. Using high-resolution live imaging in zebrafish, we show that presumptive eye cells acquire apicobasal polarity and adopt neuroepithelial character prior to other regions of the neural plate. Neur....
Categories: Biology, Journals

LDL Cholesterol Recycles to the Plasma Membrane via a Rab8a-Myosin5b-Actin-Dependent Membrane Transport Route

Developmental Cell (most read) - Thu, 2013-10-24 02:00
Kristiina Kanerva, Riikka-Liisa Uronen, Tomas Blom, Shiqian Li, Robert Bittman, Pekka Lappalainen, Johan Peränen, Graça Raposo, Elina Ikonen. Mammalian cells acquire cholesterol, a major membrane constituent, via low-density lipoprotein (LDL) uptake. However, the mechanisms by which LDL cholesterol reaches the plasma membrane (PM) have ....
Categories: Biology, Journals

Circulating Precursor CCR7loPD-1hi CXCR5+ CD4+ T Cells Indicate Tfh Cell Activity and Promote Antibody Responses upon Antigen Reexposure

Immunity (most read) - Thu, 2013-10-17 02:00
Jing He, Louis M. Tsai, Yew Ann Leong, Xin Hu, Cindy S. Ma, Nina Chevalier, Xiaolin Sun, Kirsten Vandenberg, Steve Rockman, Yan Ding, Lei Zhu, Wei Wei, Changqi Wang, Alexander Karnowski, Gabrielle T. Belz, Joanna R. Ghali, Matthew C. Cook, D. Sean Riminton, André Veillette, Pamela L. Schwartzberg, Fabienne Mackay, Robert Brink, Stuart G. Tangye, Carola G. Vinuesa, Charles R. Mackay, Zhanguo Li, Di Yu. Follicular B helper T (Tfh) cells support high affinity and long-term antibody responses. Here we found that within circulating CXCR5+ CD4+ T cells in humans and mice, the CC....

Spatiotemporal Dynamics of Intratumoral Immune Cells Reveal the Immune Landscape in Human Cancer

Immunity (most read) - Thu, 2013-10-17 02:00
Gabriela Bindea, Bernhard Mlecnik, Marie Tosolini, Amos Kirilovsky, Maximilian Waldner, Anna C. Obenauf, Helen Angell, Tessa Fredriksen, Lucie Lafontaine, Anne Berger, Patrick Bruneval, Wolf Herman Fridman, Christoph Becker, Franck Pagès, Michael R. Speicher, Zlatko Trajanoski, Jérôme Galon. The complex interactions between tumors and their microenvironment remain to be elucidated. Combining large-scale approaches, we examined the spatio-temporal dynamics of 28 different immune cell t....

Th9 Cells Drive Host Immunity against Gastrointestinal Worm Infection

Immunity (most read) - Thu, 2013-10-17 02:00
Paula Licona-Limón, Jorge Henao-Mejia, Angela U. Temann, Nicola Gagliani, Ileana Licona-Limón, Harumichi Ishigame, Liming Hao, De’Broski R. Herbert, Richard A. Flavell. Type 2 inflammatory cytokines, including interleukin-4 (IL-4), IL-5, IL-9, and IL-13, drive the characteristic features of immunity against parasitic worms and allergens. Whether IL-9 serves an es....

Blood Tfh Cells Come with Colors

Immunity (most read) - Thu, 2013-10-17 02:00
Nathalie Schmitt, Hideki Ueno. Blood CXCR5+ CD4+ T cells share phenotypic and functional similarities with T follicular helper cells. Studies by He et al. (2013) and Locci et al. (2013) in this issue of

A “Big Data” View of the Tumor “Immunome”

Immunity (most read) - Thu, 2013-10-17 02:00
Nicholas P. Restifo. Tumors are complex masses containing not just neoplastic cells but also stromal cells, neovasculature, and a gamut of immune cells. In this issue, Bindea et al. (2013) identify a surprising new “i....

Arginine Methylation Modulates Autophagic Degradation of PGL Granules in C. elegans

Molecular Cell (most read) - Thu, 2013-10-17 02:00
Sihui Li, Peiguo Yang, E Tian, Hong Zhang. The selective degradation of intracellular components by autophagy involves sequential interactions of the cargo with a receptor, which also binds the autophagosomal protein Atg8 and a scaffold pr....

Stochastic ERK Activation Induced by Noise and Cell-to-Cell Propagation Regulates Cell Density-Dependent Proliferation

Molecular Cell (most read) - Thu, 2013-10-17 02:00
Kazuhiro Aoki, Yuka Kumagai, Atsuro Sakurai, Naoki Komatsu, Yoshihisa Fujita, Clara Shionyu, Michiyuki Matsuda. The extracellular signal-regulated kinase (ERK) plays a central role in the signaling cascades of cell growth. Here, we show that stochastic ERK activity pulses regulate cell proliferation rates i....

VprBP Has Intrinsic Kinase Activity Targeting Histone H2A and Represses Gene Transcription

Molecular Cell (most read) - Thu, 2013-10-17 02:00
Kyunghwan Kim, Jin-Man Kim, Joong-Sun Kim, Jongkyu Choi, Yong Suk Lee, Nouri Neamati, Jin Sook Song, Kyu Heo, Woojin An. Histone modifications play important roles in the regulation of gene expression and chromatin organization. VprBP has been implicated in transcriptionally silent chromatin formation and cell-cycle....

Transplantation of Expanded Fetal Intestinal Progenitors Contributes to Colon Regeneration after Injury

Stem Cell: most read - Thu, 2013-10-17 02:00
Robert P. Fordham, Shiro Yui, Nicholas R.F. Hannan, Christoffer Soendergaard, Alison Madgwick, Pawel J. Schweiger, Ole H. Nielsen, Ludovic Vallier, Roger A. Pedersen, Tetsuya Nakamura, Mamoru Watanabe, Kim B. Jensen. Regeneration and homeostasis in the adult intestinal epithelium is driven by proliferative resident stem cells, whose functional properties during organismal development are largely unknown. Here,....
Categories: Biology, Journals, Stem Cells

PTP1B Is an Effector of Activin Signaling and Regulates Neural Specification of Embryonic Stem Cells

Stem Cell: most read - Thu, 2013-10-17 02:00
Kamil Matulka, Hsuan-Hwai Lin, Hana Hříbková, Dafe Uwanogho, Petr Dvořák, Yuh-Man Sun. During embryogenesis, the Activin/Nodal pathway promotes the mesendodermal lineage and inhibits neural fate. The molecular mechanisms underlying this role of the Activin/Nodal pathway are not clea....
Categories: Biology, Journals, Stem Cells