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Origins and Functional Specialization of Macrophages and of Conventional and Monocyte-Derived Dendritic Cells in Mouse Skin

Immunity (most read) - Thu, 2013-10-31 02:00
Samira Tamoutounour, Martin Guilliams, Frédéric Montanana Sanchis, Hong Liu, Dorothea Terhorst, Camille Malosse, Emeline Pollet, Laurence Ardouin, Hervé Luche, Cindy Sanchez, Marc Dalod, Bernard Malissen, Sandrine Henri. In the skin, the lack of markers permitting the unambiguous identification of macrophages and of conventional and monocyte-derived dendritic cells (DCs) complicates understanding of their contribu....

Stable Pausing by RNA Polymerase II Provides an Opportunity to Target and Integrate Regulatory Signals

Molecular Cell (most read) - Thu, 2013-10-31 02:00
Telmo Henriques, Daniel A. Gilchrist, Sergei Nechaev, Michael Bern, Ginger W. Muse, Adam Burkholder, David C. Fargo, Karen Adelman. Metazoan gene expression is often regulated after the recruitment of RNA polymerase II (Pol II) to promoters, through the controlled release of promoter-proximally paused Pol II into productive RN....

Targeting Mitochondrial DNA with a Platinum-Based Anticancer Agent

Chemistry and Biology (most read) - Thu, 2013-10-31 02:00
Simon P. Wisnovsky, Justin J. Wilson, Robert J. Radford, Mark P. Pereira, Maria R. Chan, Rebecca R. Laposa, Stephen J. Lippard, Shana O. Kelley. An analog of the anticancer drug cisplatin (mtPt) was delivered to mitochondria of human cells using a peptide specifically targeting this organelle. mtPt induces apoptosis without damaging nuclea....
Categories: Biology, Chemistry, Journals

Identification of EZH2 and EZH1 Small Molecule Inhibitors with Selective Impact on Diffuse Large B Cell Lymphoma Cell Growth

Chemistry and Biology (most read) - Thu, 2013-10-31 02:00
Shivani Garapaty-Rao, Christopher Nasveschuk, Alexandre Gagnon, Eric Y. Chan, Peter Sandy, Jennifer Busby, Srividya Balasubramanian, Robert Campbell, Feng Zhao, Louise Bergeron, James E. Audia, Brian K. Albrecht, Jean-Christophe Harmange, Richard Cummings, Patrick Trojer. The histone methyltransferase enhancer of Zeste homolog 2 (EZH2) is a candidate oncogene due to its prevalent overexpression in malignant diseases, including late stage prostate and breast cancers....
Categories: Biology, Chemistry, Journals

Next-Generation NAMPT Inhibitors Identified by Sequential High-Throughput Phenotypic Chemical and Functional Genomic Screens

Chemistry and Biology (most read) - Thu, 2013-10-31 02:00
Christina J. Matheny, Michael C. Wei, Michael C. Bassik, Alicia J. Donnelly, Martin Kampmann, Masayuki Iwasaki, Obdulio Piloto, David E. Solow-Cordero, Donna M. Bouley, Rachel Rau, Patrick Brown, Michael T. McManus, Jonathan S. Weissman, Michael L. Cleary. Phenotypic high-throughput chemical screens allow for discovery of small molecules that modulate complex phenotypes and provide lead compounds for novel therapies; however, identification of the m....
Categories: Biology, Chemistry, Journals

Cyanobacterial Polyketide Synthase Docking Domains: A Tool for Engineering Natural Product Biosynthesis

Chemistry and Biology (most read) - Thu, 2013-10-31 02:00
Jonathan R. Whicher, Sarah S. Smaga, Douglas A. Hansen, William C. Brown, William H. Gerwick, David H. Sherman, Janet L. Smith. Modular type I polyketide synthases (PKSs) are versatile biosynthetic systems that initiate, successively elongate, and modify acyl chains. Intermediate transfer between modules is mediated via do....
Categories: Biology, Chemistry, Journals

Luminal Mitosis Drives Epithelial Cell Dispersal within the Branching Ureteric Bud

Developmental Cell (most read) - Thu, 2013-10-31 02:00
Adam Packard, Kylie Georgas, Odyssé Michos, Paul Riccio, Cristina Cebrian, Alexander N. Combes, Adler Ju, Anna Ferrer-Vaquer, Anna-Katerina Hadjantonakis, Hui Zong, Melissa H. Little, Frank Costantini. The ureteric bud is an epithelial tube that undergoes branching morphogenesis to form the renal collecting system. Although development of a normal kidney depends on proper ureteric bud morphogene....
Categories: Biology, Journals

EpCAM Controls Actomyosin Contractility and Cell Adhesion by Direct Inhibition of PKC

Developmental Cell (most read) - Thu, 2013-10-31 02:00
Nadim Maghzal, Hulya A. Kayali, Nazanin Rohani, Andrey V. Kajava, François Fagotto. Epithelial cell adhesion molecule (EpCAM) is a cell-surface protein highly expressed in embryonic tissues and in malignant carcinomas. We report that EpCAM acts as a potent inhibitor of novel prot....
Categories: Biology, Journals

External Mechanical Cues Trigger the Establishment of the Anterior-Posterior Axis in Early Mouse Embryos

Developmental Cell (most read) - Mon, 2013-10-28 02:00
Ryuji Hiramatsu, Toshiki Matsuoka, Chiharu Kimura-Yoshida, Sung-Woong Han, Kyoko Mochida, Taiji Adachi, Shuichi Takayama, Isao Matsuo. Mouse anterior-posterior axis polarization is preceded by formation of the distal visceral endoderm (DVE) by unknown mechanisms. Here, we show by in vitro culturing of embryos immediately after im....
Categories: Biology, Journals

Retraction Notice to: Dystroglycan and Perlecan Provide a Basal Cue Required for Epithelial Polarity during Energetic Stress

Developmental Cell (most read) - Mon, 2013-10-28 02:00
Vincent Mirouse, Christina P. Christoforou, Cornelia Fritsch, Daniel St Johnston, Robert P. Ray.
Categories: Biology, Journals

Translation Repressors, an RNA Helicase, and Developmental Cues Control RNP Phase Transitions during Early Development

Developmental Cell (most read) - Mon, 2013-10-28 02:00
Arnaud Hubstenberger, Scott L. Noble, Cristiana Cameron, Thomas C. Evans. Like membranous organelles, large-scale coassembly of macromolecules can organize functions in cells. Ribonucleoproteins (RNPs) can form liquid or solid aggregates, but control and consequences of....
Categories: Biology, Journals

Cilia Grow by Taking a Bite out of the Cell

Developmental Cell (most read) - Mon, 2013-10-28 02:00
Nathan W. Pierce, Maxence V. Nachury. Autophagy and primary cilium assembly have long been known to be induced by the same conditions in cultured cells. Two recent studies in Nature—Tang et al. (2013) and Pampliega et al. (2013....
Categories: Biology, Journals

Cleavage of TFIIA by Taspase1 Activates TRF2-Specified Mammalian Male Germ Cell Programs

Developmental Cell (most read) - Mon, 2013-10-28 02:00
Toshinao Oyama, Satoru Sasagawa, Shugaku Takeda, Rex A. Hess, Paul M. Lieberman, Emily H. Cheng, James J. Hsieh. The evolution of tissue-specific general transcription factors (GTFs), such as testis-specific TBP-related factor 2 (TRF2), enables the spatiotemporal expression of highly specialized genetic prog....
Categories: Biology, Journals

A Network of Interactions Enables CCM3 and STK24 to Coordinate UNC13D-Driven Vesicle Exocytosis in Neutrophils

Developmental Cell (most read) - Mon, 2013-10-28 02:00
Yong Zhang, Wenwen Tang, Haifeng Zhang, Xiaofeng Niu, Yingke Xu, Jiasheng Zhang, Kun Gao, Weijun Pan, Titus J. Boggon, Derek Toomre, Wang Min, Dianqing Wu. Neutrophil degranulation plays an important role in acute innate immune responses and is tightly regulated because the granule contents can cause tissue damage. However, this regulation remains po....
Categories: Biology, Journals

A Beneficial Role for Immunoglobulin E in Host Defense against Honeybee Venom

Immunity (most read) - Thu, 2013-10-24 02:00
Thomas Marichal, Philipp Starkl, Laurent L. Reber, Janet Kalesnikoff, Hans C. Oettgen, Mindy Tsai, Martin Metz, Stephen J. Galli. Allergies are widely considered to be misdirected type 2 immune responses, in which immunoglobulin E (IgE) antibodies are produced against any of a broad range of seemingly harmless antigens. Howe....

Bee Venom Phospholipase A2 Induces a Primary Type 2 Response that Is Dependent on the Receptor ST2 and Confers Protective Immunity

Immunity (most read) - Thu, 2013-10-24 02:00
Noah W. Palm, Rachel K. Rosenstein, Shuang Yu, Dominik D. Schenten, Esther Florsheim, Ruslan Medzhitov. Venoms consist of toxic components that are delivered to their victims via bites or stings. Venoms also represent a major class of allergens in humans. Phospholipase A2 (PLA2) is a conserved compo....

PrimPol, an Archaic Primase/Polymerase Operating in Human Cells

Molecular Cell (most read) - Thu, 2013-10-24 02:00
Sara García-Gómez, Aurelio Reyes, María I. Martínez-Jiménez, E. Sandra Chocrón, Silvana Mourón, Gloria Terrados, Christopher Powell, Eduardo Salido, Juan Méndez, Ian J. Holt, Luis Blanco. We describe a second primase in human cells, PrimPol, which has the ability to start DNA chains with deoxynucleotides unlike regular primases, which use exclusively ribonucleotides. Moreover, Prim....

The DNA Translocase FANCM/MHF Promotes Replication Traverse of DNA Interstrand Crosslinks

Molecular Cell (most read) - Thu, 2013-10-24 02:00
Jing Huang, Shuo Liu, Marina A. Bellani, Arun Kalliat Thazhathveetil, Chen Ling, Johan P. de Winter, Yinsheng Wang, Weidong Wang, Michael M. Seidman. The replicative machinery encounters many impediments, some of which can be overcome by lesion bypass or replication restart pathways, leaving repair for a later time. However, interstrand crossli....

Braking BRAF: AMPK Leaves ERK Stranded in the Desert

Molecular Cell (most read) - Thu, 2013-10-24 02:00
Matthew Martin, Richard Marais. In this issue, Shen et al. (2013) show that BRAF phosphorylation by AMPK attenuates BRAF signaling and inhibits cell proliferation. This identifies BRAF as a novel AMPK substrate and further revea....

Design, Synthesis, and Biological Evaluation of an Allosteric Inhibitor of HSET that Targets Cancer Cells with Supernumerary Centrosomes

Chemistry and Biology (most read) - Thu, 2013-10-24 02:00
Ciorsdaidh A. Watts, Frances M. Richards, Andreas Bender, Peter J. Bond, Oliver Korb, Oliver Kern, Michelle Riddick, Paul Owen, Rebecca M. Myers, Jordan Raff, Fanni Gergely, Duncan I. Jodrell, Steven V. Ley. Centrosomes associate with spindle poles; thus, the presence of two centrosomes promotes bipolar spindle assembly in normal cells. Cancer cells often contain supernumerary centrosomes, and to avoi....
Categories: Biology, Chemistry, Journals