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Exploring Intermolecular Interactions of a Substrate Binding Protein Using a Riboswitch-Based Sensor

Chemistry and Biology (most read) - Wed, 2013-11-27 02:00
Casey C. Fowler, Seiji Sugiman-Marangos, Murray S. Junop, Eric D. Brown, Yingfu Li. The study of biological transporters can be hampered by a dearth of methodology for tracking their activity within cells. Here, we present a means of monitoring the function of transport machinery....
Categories: Biology, Chemistry, Journals

Neonatal Fc Receptor Expression in Dendritic Cells Mediates Protective Immunity against Colorectal Cancer

Immunity (most read) - Wed, 2013-11-27 02:00
Kristi Baker, Timo Rath, Magdalena B. Flak, Janelle C. Arthur, Zhangguo Chen, Jonathan N. Glickman, Inti Zlobec, Eva Karamitopoulou, Matthew D. Stachler, Robert D. Odze, Wayne I. Lencer, Christian Jobin, Richard S. Blumberg. Cancers arising in mucosal tissues account for a disproportionately large fraction of malignancies. Immunoglobulin G (IgG) and the neonatal Fc receptor for IgG (FcRn) have an important function in....

Deubiquitinating Enzyme Specificity for Ubiquitin Chain Topology Profiled by Di-Ubiquitin Activity Probes

Chemistry and Biology (most read) - Wed, 2013-11-27 02:00
Joanna F. McGouran, Selina R. Gaertner, Mikael Altun, Holger B. Kramer, Benedikt M. Kessler. Posttranslational modification with ubiquitin (Ub) controls many cellular processes, and aberrant ubiquitination can contribute to cancer, immunopathology, and neurodegeneration. The versatility a....
Categories: Biology, Chemistry, Journals

A Small-Molecule Inducer of PDX1 Expression Identified by High-Throughput Screening

Chemistry and Biology (most read) - Wed, 2013-11-27 02:00
Yuan Yuan, Kate Hartland, Zarko Boskovic, Yikai Wang, Deepika Walpita, Philippe A. Lysy, Cheng Zhong, Damian W. Young, Young-kwon Kim, Nicola J. Tolliday, Etienne M. Sokal, Stuart L. Schreiber, Bridget K. Wagner. Pancreatic and duodenal homeobox 1 (PDX1), a member of the homeodomain-containing transcription factor family, is a key transcription factor important for both pancreas development and mature β ce....
Categories: Biology, Chemistry, Journals

Polycomb-Dependent H3K27me1 and H3K27me2 Regulate Active Transcription and Enhancer Fidelity

Molecular Cell (most read) - Wed, 2013-11-27 02:00
Karin J. Ferrari, Andrea Scelfo, SriGanesh Jammula, Alessandro Cuomo, Iros Barozzi, Alexandra Stützer, Wolfgang Fischle, Tiziana Bonaldi, Diego Pasini. H3K27me3 is deposited at promoters by the preferential association of Polycomb repressive complex 2 (PRC2) with CpG-rich DNA elements regulating development by repressing gene transcription. H3K27....

Sensory-Neuron Subtype-Specific Transcriptional Programs Controlling Dendrite Morphogenesis: Genome-wide Analysis of Abrupt and Knot/Collier

Developmental Cell (most read) - Wed, 2013-11-27 02:00
Yukako Hattori, Tadao Usui, Daisuke Satoh, Sanefumi Moriyama, Kohei Shimono, Takehiko Itoh, Katsuhiko Shirahige, Tadashi Uemura. The transcription factors Abrupt (Ab) and Knot (Kn) act as selectors of distinct dendritic arbor morphologies in two classes of Drosophila sensory neurons, termed class I and class IV, resp....
Categories: Biology, Journals

Control of Epithelial Cell Migration and Invasion by the IKKβ- and CK1α-Mediated Degradation of RAPGEF2

Developmental Cell (most read) - Wed, 2013-11-27 02:00
Roberto Magliozzi, Teck Yew Low, Bart G.M.W. Weijts, Tianhong Cheng, Emma Spanjaard, Shabaz Mohammed, Anouk van Veen, Huib Ovaa, Johan de Rooij, Fried J.T. Zwartkruis, Johannes L. Bos, Alain de Bruin, Albert J.R. Heck, Daniele Guardavaccaro. Epithelial cell migration is crucial for the development and regeneration of epithelial tissues. Aberrant regulation of epithelial cell migration has a major role in pathological processes such as....
Categories: Biology, Journals

c-Met-Dependent Multipotent Labyrinth Trophoblast Progenitors Establish Placental Exchange Interface

Developmental Cell (most read) - Mon, 2013-11-25 02:00
Masaya Ueno, Lydia K. Lee, Akanksha Chhabra, Yeon Joo Kim, Rajkumar Sasidharan, Ben Van Handel, Ying Wang, Masakazu Kamata, Paniz Kamran, Konstantina-Ioanna Sereti, Reza Ardehali, Meisheng Jiang, Hanna K.A. Mikkola. The placenta provides the interface for gas and nutrient exchange between the mother and the fetus. Despite its critical function in sustaining pregnancy, the stem/progenitor cell hierarchy and mo....
Categories: Biology, Journals

Side-Chain Modification of Cytokinins Controls Shoot Growth in Arabidopsis

Developmental Cell (most read) - Mon, 2013-11-25 02:00
Takatoshi Kiba, Kentaro Takei, Mikiko Kojima, Hitoshi Sakakibara. Cytokinins (CKs), a class of plant hormones, are central regulators of plant growth and development. Based on numerous physiological and genetic studies, the quantitative regulation of cytokinin l....
Categories: Biology, Journals

Entrapment of Chromosomes by Condensin Rings Prevents Their Breakage during Cytokinesis

Developmental Cell (most read) - Mon, 2013-11-25 02:00
Sara Cuylen, Jutta Metz, Andrea Hruby, Christian H. Haering. Successful segregation of chromosomes during mitosis and meiosis depends on the action of the ring-shaped condensin complex, but how condensin ensures the complete disjunction of sister chromatids....
Categories: Biology, Journals

Loss of dE2F Compromises Mitochondrial Function

Developmental Cell (most read) - Mon, 2013-11-25 02:00
Aaron M. Ambrus, Abul B.M.M.K. Islam, Katherine B. Holmes, Nam Sung Moon, Nuria Lopez-Bigas, Elizaveta V. Benevolenskaya, Maxim V. Frolov. E2F/DP transcription factors regulate cell proliferation and apoptosis. Here, we investigated the mechanism of the resistance of Drosophila dDP mutants to irradiation-induced apoptosis. Con....
Categories: Biology, Journals

A Twoferase for Lipid Transfer at ER-Golgi Contact Sites

Developmental Cell (most read) - Mon, 2013-11-25 02:00
Tim P. Levine. Reporting in Cell, Mesmin et al. (2013) show how a single molecule of oxysterol binding protein, which has a lipid binding domain that solubilizes both sterol and PI4P, might integrate its ....
Categories: Biology, Journals

Endogenous Nitrated Nucleotide Is a Key Mediator of Autophagy and Innate Defense against Bacteria

Molecular Cell (most read) - Thu, 2013-11-21 02:00
Chiaki Ito, Yohei Saito, Takashi Nozawa, Shigemoto Fujii, Tomohiro Sawa, Hirofumi Inoue, Tetsuro Matsunaga, Shahzada Khan, Soichiro Akashi, Ryota Hashimoto, Chihiro Aikawa, Eriko Takahashi, Hiroshi Sagara, Masaaki Komatsu, Keiji Tanaka, Takaaki Akaike, Ichiro Nakagawa, Hirokazu Arimoto. Autophagy is a cellular self-catabolic process wherein organelles, macromolecules, and invading microbes are sequestered in autophagosomes that fuse with lysosomes. In this study, we uncover the r....

Deacetylase-Independent Function of HDAC3 in Transcription and Metabolism Requires Nuclear Receptor Corepressor

Molecular Cell (most read) - Thu, 2013-11-21 02:00
Zheng Sun, Dan Feng, Bin Fang, Shannon E. Mullican, Seo-Hee You, Hee-Woong Lim, Logan J. Everett, Christopher S. Nabel, Yun Li, Vignesh Selvakumaran, Kyoung-Jae Won, Mitchell A. Lazar. Histone deacetylases (HDACs) are believed to regulate gene transcription by catalyzing deacetylation reactions. HDAC3 depletion in mouse liver upregulates lipogenic genes and results in severe hep....

Proofreading of Noncognate Acyl Adenylates by an Acyl-Coenzyme A Ligase

Chemistry and Biology (most read) - Thu, 2013-11-21 02:00
Miglena Manandhar, John E. Cronan. Aminoacyl-tRNA synthetases remove (proofread) incorrect substrates and thereby prevent errors in protein synthesis. We report enzyme-catalyzed pretransfer editing by pimeloyl-coenzyme A (CoA) liga....
Categories: Biology, Chemistry, Journals

Core Assembly Mechanism of Quinocarcin/SF-1739: Bimodular Complex Nonribosomal Peptide Synthetases for Sequential Mannich-type Reactions

Chemistry and Biology (most read) - Thu, 2013-11-21 02:00
Tomoshige Hiratsuka, Kento Koketsu, Atsushi Minami, Shunsuke Kaneko, Chiaki Yamazaki, Kenji Watanabe, Hiroki Oguri, Hideaki Oikawa. Quinocarcin and SF-1739, potent antitumor antibiotics, share a common tetracyclic tetrahydroisoquinoline (THIQ)-pyrrolidine core scaffold. Herein, we describe the identification of their biosynthe....
Categories: Biology, Chemistry, Journals

The Capsids of HIV-1 and HIV-2 Determine Immune Detection of the Viral cDNA by the Innate Sensor cGAS in Dendritic Cells

Immunity (most read) - Thu, 2013-11-21 02:00
Xavier Lahaye, Takeshi Satoh, Matteo Gentili, Silvia Cerboni, Cécile Conrad, Ilse Hurbain, Ahmed El Marjou, Christine Lacabaratz, Jean-Daniel Lelièvre, Nicolas Manel. HIV-2 is less pathogenic for humans than HIV-1 and might provide partial cross-protection from HIV-1-induced pathology. Although both viruses replicate in the T cells of infected patients, only HI....

Enhanced Telomere Rejuvenation in Pluripotent Cells Reprogrammed via Nuclear Transfer Relative to Induced Pluripotent Stem Cells

Stem Cell: most read - Thu, 2013-11-21 02:00
Rongrong Le, Zhaohui Kou, Yonghua Jiang, Ming Li, Bo Huang, Wenqiang Liu, Hui Li, Xiaochen Kou, Wanzhong He, Karl Lenhard Rudolph, Zhenyu Ju, Shaorong Gao. Although somatic cell nuclear transfer (SCNT) and induction of pluripotency (to form iPSCs) are both recognized reprogramming methods, there has been relatively little comparative analysis of the ....
Categories: Biology, Journals, Stem Cells

Contractile Forces Sustain and Polarize Hematopoiesis from Stem and Progenitor Cells

Stem Cell: most read - Thu, 2013-11-21 02:00
Jae-Won Shin, Amnon Buxboim, Kyle R. Spinler, Joe Swift, David A. Christian, Christopher A. Hunter, Catherine Léon, Christian Gachet, P.C. Dave P. Dingal, Irena L. Ivanovska, Florian Rehfeldt, Joel Anne Chasis, Dennis E. Discher. Self-renewal and differentiation of stem cells depend on asymmetric division and polarized motility processes that in other cell types are modulated by nonmuscle myosin-II (MII) forces and matrix ....
Categories: Biology, Journals, Stem Cells

Fundamental Differences in Dedifferentiation and Stem Cell Recruitment during Skeletal Muscle Regeneration in Two Salamander Species

Stem Cell: most read - Thu, 2013-11-21 02:00
Tatiana Sandoval-Guzmán, Heng Wang, Shahryar Khattak, Maritta Schuez, Kathleen Roensch, Eugeniu Nacu, Akira Tazaki, Alberto Joven, Elly M. Tanaka, András Simon. Salamanders regenerate appendages via a progenitor pool called the blastema. The cellular mechanisms underlying regeneration of muscle have been much debated but have remained unclear. Here we app....
Categories: Biology, Journals, Stem Cells