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Genetic Modification and Screening in Rat Using Haploid Embryonic Stem Cells

Stem Cell: most read - Thu, 2013-12-19 02:00
Wei Li, Xin Li, Tianda Li, Ming-Gui Jiang, Haifeng Wan, Guan-Zheng Luo, Chunjing Feng, Xiaolong Cui, Fei Teng, Yan Yuan, Quan Zhou, Qi Gu, Ling Shuai, Jiahao Sha, Yamei Xiao, Liu Wang, Zhonghua Liu, Xiu-Jie Wang, Xiao-Yang Zhao, Qi Zhou. The rat is an important animal model in biomedical research, but practical limitations to genetic manipulation have restricted the application of genetic analysis. Here we report the derivation of....
Categories: Biology, Journals, Stem Cells

Fluorescent Probes for Lipid Rafts: From Model Membranes to Living Cells

Chemistry and Biology (most read) - Thu, 2013-12-19 02:00
Andrey S. Klymchenko, Rémy Kreder. Membrane microdomains (rafts) remain one of the controversial issues in biophysics. Fluorescent molecular probes, which make these lipid nanostructures visible through optical techniques, are one ....
Categories: Biology, Chemistry, Journals

Structure and Function of Phosphonoacetaldehyde Dehydrogenase: The Missing Link in Phosphonoacetate Formation

Chemistry and Biology (most read) - Thu, 2013-12-19 02:00
Vinayak Agarwal, Spencer C. Peck, Jui-Hui Chen, Svetlana A. Borisova, Jonathan R. Chekan, Wilfred A. van der Donk, Satish K. Nair. Phosphonates (C-PO32−) have applications as antibiotics, herbicides, and detergents. In some environments, these molecules represent the predominant source of phosphorus, and....
Categories: Biology, Chemistry, Journals

Old Molecules, New Biochemistry

Chemistry and Biology (most read) - Thu, 2013-12-19 02:00
Susanne Fetzner, Steffen L. Drees. The study by Dulcey and colleagues in this issue of Chemistry & Biology changes our perception of the pathway of 2-alkyl-4-hydroxyquinoline biosynthesis by the opportunistic pathogen
Categories: Biology, Chemistry, Journals

Cyclolization of D-Lysergic Acid Alkaloid Peptides

Chemistry and Biology (most read) - Thu, 2013-12-19 02:00
Judith Havemann, Dominik Vogel, Bernhard Loll, Ullrich Keller. The tripeptide chains of the ergopeptines, a class of pharmacologically important D-lysergic acid alkaloid peptides, are arranged in a unique bicyclic cyclol based on an amino-terminal α-hydroxyam....
Categories: Biology, Chemistry, Journals

The C. elegans CSR-1 Argonaute Pathway Counteracts Epigenetic Silencing to Promote Germline Gene Expression

Developmental Cell (most read) - Thu, 2013-12-19 02:00
Meetu Seth, Masaki Shirayama, Weifeng Gu, Takao Ishidate, Darryl Conte, Craig C. Mello. Organisms can develop adaptive sequence-specific immunity by reexpressing pathogen-specific small RNAs that guide gene silencing. For example, the C. elegans PIWI-Argonaute/piwi-interacting....
Categories: Biology, Journals

Protection of Germline Gene Expression by the C. elegans Argonaute CSR-1

Developmental Cell (most read) - Thu, 2013-12-19 02:00
Christopher J. Wedeles, Monica Z. Wu, Julie M. Claycomb. In Caenorhabditis elegans, the Piwi-interacting small RNA (piRNA)-mediated germline surveillance system encodes more than 30,000 unique 21-nucleotide piRNAs, which silence a variety of fore....
Categories: Biology, Journals

Insights into the structure of class B GPCRs

Trends in Pharmacological Sciences - Thu, 2013-12-19 02:00
Kaspar Hollenstein, Chris de Graaf, Andrea Bortolato, Ming-Wei Wang, Fiona H. Marshall, Raymond C. Stevens.
• GCGR and CRF1 structures show different features compared to class A GPCRs.
• Class B structures and structure-based drug discovery for peptide hormone G....
Categories: Biology, Healthcare, Journals

Molecularly targeted cancer therapy: some lessons from the past decade

Trends in Pharmacological Sciences - Thu, 2013-12-19 02:00
Min Huang, Aijun Shen, Jian Ding, Meiyu Geng.
• Tumor heterogeneity is the key factor underlying limited response rate to targeted therapy.
• Personalized medicines depend on biomarkers for selecting patients and....
Categories: Biology, Healthcare, Journals

Hepatic RIG-I Predicts Survival and Interferon-α Therapeutic Response in Hepatocellular Carcinoma

Cancer Cell - Thu, 2013-12-19 02:00
Jin Hou, Ye Zhou, Yuanyuan Zheng, Jia Fan, Weiping Zhou, Irene O.L. Ng, Huichuan Sun, Lunxiu Qin, Shuangjian Qiu, Joyce M.F. Lee, Chung-Mau Lo, Kwan Man, Yuan Yang, Yun Yang, Yingyun Yang, Qian Zhang, Xuhui Zhu, Nan Li, Zhengxin Wang, Guoshan Ding, Shi-Mei Zhuang, Limin Zheng, Xiaoling Luo, Yuan Xie, Anmin Liang, Zhugang Wang, Ming Zhang, Qiang Xia, Tingbo Liang, Yizhi Yu, Xuetao Cao. In hepatocellular carcinoma (HCC), biomarkers for prediction of prognosis and response to immunotherapy such as interferon-α (IFN-α) would be very useful in the clinic. We found that expression of....
Categories: Biology, Cancer, Journals

Computer Use Changes Generalization of Movement Learning

Current Biology - Thu, 2013-12-19 02:00
Kunlin Wei, Xiang Yan, Gaiqing Kong, Cong Yin, Fan Zhang, Qining Wang, Konrad Paul Kording. Over the past few decades, one of the most salient lifestyle changes for us has been the use of computers. For many of us, manual interaction with a computer occupies a large portion of our workin....
Categories: Biology, Journals

The Availability of Research Data Declines Rapidly with Article Age

Current Biology - Thu, 2013-12-19 02:00
Timothy H. Vines, Arianne Y.K. Albert, Rose L. Andrew, Florence Débarre, Dan G. Bock, Michelle T. Franklin, Kimberly J. Gilbert, Jean-Sébastien Moore, Sébastien Renaut, Diana J. Rennison. Policies ensuring that research data are available on public archives are increasingly being implemented at the government [1], funding agency [2–4], and journal [5, 6] level. These policies are p....
Categories: Biology, Journals

Rickettsia Actin-Based Motility Occurs in Distinct Phases Mediated by Different Actin Nucleators

Current Biology - Thu, 2013-12-19 02:00
Shawna C.O. Reed, Rebecca L. Lamason, Viviana I. Risca, Emma Abernathy, Matthew D. Welch. Many intracellular bacterial pathogens undergo actin-based motility to promote cell-cell spread during infection [1]. For each pathogen, motility was assumed to be driven by a single actin polymer....
Categories: Biology, Journals

Autophagy in Oncogenic K-Ras Promotes Basal Extrusion of Epithelial Cells by Degrading S1P

Current Biology - Thu, 2013-12-19 02:00
Gloria Slattum, Yapeng Gu, Roger Sabbadini, Jody Rosenblatt. BackgroundTo maintain a protective barrier, epithelia extrude cells destined to die by contracting a band of actin and myosin. Although extrusion can remove cells triggered to die by apoptotic sti....
Categories: Biology, Journals

KI Motifs of Human Knl1 Enhance Assembly of Comprehensive Spindle Checkpoint Complexes around MELT Repeats

Current Biology - Thu, 2013-12-19 02:00
Veronica Krenn, Katharina Overlack, Ivana Primorac, Suzan van Gerwen, Andrea Musacchio. BackgroundThe KMN network, a ten-subunit protein complex, mediates the interaction of kinetochores with spindle microtubules and recruits spindle assembly checkpoint (SAC) constituents to halt cel....
Categories: Biology, Journals

The Representation of Social Facial Touch in Rat Barrel Cortex

Current Biology - Thu, 2013-12-19 02:00
Evgeny Bobrov, Jason Wolfe, Rajnish P. Rao, Michael Brecht. Controlled presentation of stimuli to anesthetized [1] or awake [2] animals suggested that neurons in sensory cortices respond to elementary features [3, 4], but we know little about neuronal resp....
Categories: Biology, Journals

The Interleukin-1 Family: Back to the Future

Immunity (most read) - Thu, 2013-12-12 02:00
Cecilia Garlanda, Charles A. Dinarello, Alberto Mantovani. Interleukin-1 (IL-1) is a central mediator of innate immunity and inflammation. The IL-1 family includes seven ligands with agonist activity (IL-1α and IL-1β, IL-18, IL-33, IL-36α, IL-36β, IL-36γ)....

Leonard Herzenberg (1931–2013): The Life of FACS

Immunity (most read) - Thu, 2013-12-12 02:00
Richard R. Hardy, Mario Roederer.

Thymic Stromal Lymphopoietin-Mediated Extramedullary Hematopoiesis Promotes Allergic Inflammation

Immunity (most read) - Thu, 2013-12-12 02:00
Mark C. Siracusa, Steven A. Saenz, Elia D. Tait Wojno, Brian S. Kim, Lisa C. Osborne, Carly G. Ziegler, Alain J. Benitez, Kathryn R. Ruymann, Donna L. Farber, Patrick M. Sleiman, Hakon Hakonarson, Antonella Cianferoni, Mei-Lun Wang, Jonathan M. Spergel, Michael R. Comeau, David Artis. Extramedullary hematopoiesis (EMH) refers to the differentiation of hematopoietic stem cells (HSCs) into effector cells that occurs in compartments outside of the bone marrow. Previous studies lin....

New Twist on an Ancient Innate Immune Pathway

Immunity (most read) - Thu, 2013-12-12 02:00
Stephane Lajoie, Marsha Wills-Karp. Activation of the complement system has long been known to be regulated by a series of steps involving fluid-phase convertases. In this issue of Immunity, Liszewski et al. (2013) report the....