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A Cryptic Polyene Biosynthetic Gene Cluster in Streptomyces calvus Is Expressed upon Complementation with a Functional bldA Gene

Chemistry and Biology (most read) - Thu, 2013-10-10 02:00
Lindsay Kalan, Arne Gessner, Maulik N. Thaker, Nicholas Waglechner, XiMing Zhu, Anjuli Szawiola, Andreas Bechthold, Gerard D. Wright, David L. Zechel. Streptomyces calvus is best known as the producer of the fluorinated natural product nucleocidin. This strain of Streptomycetes is also unusual for displaying a “bald” phenotype that....
Categories: Biology, Chemistry, Journals

The Folliculin Tumor Suppressor Is a GAP for the RagC/D GTPases That Signal Amino Acid Levels to mTORC1

Molecular Cell (most read) - Thu, 2013-10-03 02:00
Zhi-Yang Tsun, Liron Bar-Peled, Lynne Chantranupong, Roberto Zoncu, Tim Wang, Choah Kim, Eric Spooner, David M. Sabatini. The mTORC1 kinase is a master growth regulator that senses numerous environmental cues, including amino acids. The Rag GTPases interact with mTORC1 and signal amino acid sufficiency by promoting t....

Molecular Mechanism of Bacterial Persistence by HipA

Molecular Cell (most read) - Thu, 2013-10-03 02:00
Elsa Germain, Daniel Castro-Roa, Nikolay Zenkin, Kenn Gerdes. HipA of Escherichia coli is a eukaryote-like serine-threonine kinase that inhibits cell growth and induces persistence (multidrug tolerance). Previously, it was proposed that HipA inhibits ....

Metabolic Activation of CaMKII by Coenzyme A

Molecular Cell (most read) - Thu, 2013-10-03 02:00
Francis McCoy, Rashid Darbandi, Hoi Chang Lee, Kavitha Bharatham, Tudor Moldoveanu, Christy R. Grace, Keela Dodd, Wenwei Lin, Si-Ing Chen, Rajendra P. Tangallapally, Manabu Kurokawa, Richard E. Lee, Anang A. Shelat, Taosheng Chen, Douglas R. Green, Robert A. Harris, Sue-Hwa Lin, Rafael A. Fissore, Roger J. Colbran, Leta K. Nutt. Active metabolism regulates oocyte cell death via calcium/calmodulin-dependent protein kinase II (CaMKII)-mediated phosphorylation of caspase-2, but the link between metabolic activity and CaMKII ....

Phosphorylation of BRAF by AMPK Impairs BRAF-KSR1 Association and Cell Proliferation

Molecular Cell (most read) - Thu, 2013-10-03 02:00
Che-Hung Shen, Ping Yuan, Rolando Perez-Lorenzo, Yaqing Zhang, Sze Xian Lee, Yang Ou, John M. Asara, Lewis C. Cantley, Bin Zheng. BRAF is an oncogenic protein kinase that drives cell growth and proliferation through the MEK-ERK signaling pathway. BRAF inhibitors have demonstrated antitumor efficacy in melanoma therapy but ha....

Mesenchymal Stromal Cells: Sensors and Switchers of Inflammation

Stem Cell: most read - Thu, 2013-10-03 02:00
Maria Ester Bernardo, Willem E. Fibbe. In addition to their stem/progenitor properties, mesenchymal stromal cells (MSCs) possess broad immunoregulatory properties that are being investigated for potential clinical application in treati....
Categories: Biology, Journals, Stem Cells

HIF-2α Protects Human Hematopoietic Stem/Progenitors and Acute Myeloid Leukemic Cells from Apoptosis Induced by Endoplasmic Reticulum Stress

Stem Cell: most read - Thu, 2013-10-03 02:00
Kevin Rouault-Pierre, Lourdes Lopez-Onieva, Katie Foster, Fernando Anjos-Afonso, Isabelle Lamrissi-Garcia, Martin Serrano-Sanchez, Richard Mitter, Zoran Ivanovic, Hubert de Verneuil, John Gribben, David Taussig, Hamid Reza Rezvani, Frédéric Mazurier, Dominique Bonnet. Hematopoietic stem and progenitor cells (HSPCs) are exposed to low levels of oxygen in the bone marrow niche, and hypoxia-inducible factors (HIFs) are the main regulators of cellular responses to ....
Categories: Biology, Journals, Stem Cells

Induction of Multipotential Hematopoietic Progenitors from Human Pluripotent Stem Cells via Respecification of Lineage-Restricted Precursors

Stem Cell: most read - Thu, 2013-10-03 02:00
Sergei Doulatov, Linda T. Vo, Stephanie S. Chou, Peter G. Kim, Natasha Arora, Hu Li, Brandon K. Hadland, Irwin D. Bernstein, James J. Collins, Leonard I. Zon, George Q. Daley. Human pluripotent stem cells (hPSCs) represent a promising source of patient-specific cells for disease modeling, drug screens, and cellular therapies. However, the inability to derive engraftable....
Categories: Biology, Journals, Stem Cells

Mobilized Adult Pituitary Stem Cells Contribute to Endocrine Regeneration in Response to Physiological Demand

Stem Cell: most read - Thu, 2013-10-03 02:00
Karine Rizzoti, Haruhiko Akiyama, Robin Lovell-Badge. Pituitary hormone deficiencies, with Growth Hormone deficiency being most frequent (1 in 3,500-10,000 births), cause significant morbidity. Regeneration of missing endocrine cells would be a signi....
Categories: Biology, Journals, Stem Cells

An Engineered Monomeric Zoanthus sp. Yellow Fluorescent Protein

Chemistry and Biology (most read) - Thu, 2013-10-03 02:00
Hiofan Hoi, Elizabeth S. Howe, Yidan Ding, Wei Zhang, Michelle A. Baird, Brittney R. Sell, John R. Allen, Michael W. Davidson, Robert E. Campbell. Protein engineering has created a palette of monomeric fluorescent proteins (FPs), but there remains an ∼30 nm spectral gap between the most red-shifted useful Aequorea victoria green FP (G....
Categories: Biology, Chemistry, Journals

A Role for a Dioxygenase in Auxin Metabolism and Reproductive Development in Rice

Developmental Cell (most read) - Thu, 2013-10-03 02:00
Zhigang Zhao, Yunhui Zhang, Xi Liu, Xin Zhang, Shichang Liu, Xiaowen Yu, Yulong Ren, Xiaomin Zheng, Kunneng Zhou, Ling Jiang, Xiuping Guo, Ying Gai, Chuanyin Wu, Huqu Zhai, Haiyang Wang, Jianmin Wan. Indole-3-acetic acid (IAA), the natural auxin in plants, regulates many aspects of plant growth and development. Extensive analyses have elucidated the components of auxin biosynthesis, transport,....
Categories: Biology, Journals

Control of T Helper 2 Responses by Transcription Factor IRF4-Dependent Dendritic Cells

Immunity (most read) - Thu, 2013-09-26 02:00
Yan Gao, Simone A. Nish, Ruoyi Jiang, Lin Hou, Paula Licona-Limón, Jason S. Weinstein, Hongyu Zhao, Ruslan Medzhitov. CD4+ T cell differentiation is regulated by specialized antigen-presenting cells. Dendritic cells (DCs) produce cytokines that promote naive CD4+ T cell differentiation into ....

CD301b+ Dermal Dendritic Cells Drive T Helper 2 Cell-Mediated Immunity

Immunity (most read) - Thu, 2013-09-26 02:00
Yosuke Kumamoto, Melissa Linehan, Jason S. Weinstein, Brian J. Laidlaw, Joseph E. Craft, Akiko Iwasaki. Unlike other types of T helper (Th) responses, whether the development of Th2 cells requires instruction from particular subset of dendritic cells (DCs) remains unclear. By using an in vivo deplet....

Transforming Growth Factor-β Signaling Controls the Formation and Maintenance of Gut-Resident Memory T Cells by Regulating Migration and Retention

Immunity (most read) - Thu, 2013-09-26 02:00
Nu Zhang, Michael J. Bevan. Tissue-resident memory T (Trm) cells represent a population of memory CD8+ T cells that can act as first responders to local infection. The mechanisms regulating the formation and maint....

Cooperative Control of Holliday Junction Resolution and DNA Repair by the SLX1 and MUS81-EME1 Nucleases

Molecular Cell (most read) - Thu, 2013-09-26 02:00
Dennis Castor, Nidhi Nair, Anne-Cécile Déclais, Christophe Lachaud, Rachel Toth, Thomas J. Macartney, David M.J. Lilley, J.Simon C. Arthur, John Rouse. Holliday junctions (HJs) are X-shaped DNA structures that arise during homologous recombination, which must be removed to enable chromosome segregation. The SLX1 and MUS81-EME1 nucleases can both ....