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Has FLAT fallen flat?

Trends in Pharmacological Sciences - Mon, 2014-01-06 02:00
Christopher J. Fowler.
Categories: Biology, Healthcare, Journals

Drugging unconventional targets: insights from Huntington's disease

Trends in Pharmacological Sciences - Fri, 2014-01-03 02:00
Shenliang Yu, Yijian Liang, James Palacino, Marian Difiglia, Boxun Lu.
• Lowering protein levels provides an alternative approach for unconventional targets.
• Interferon–NUB1–KLHL22 may target mutant HTT for proteasomal degradation.
Categories: Biology, Healthcare, Journals

Ubiquitin Ligase Trapping Identifies an SCFSaf1 Pathway Targeting Unprocessed Vacuolar/Lysosomal Proteins

Molecular Cell (most read) - Thu, 2014-01-02 02:00
Kevin G. Mark, Marco Simonetta, Alessio Maiolica, Charles A. Seller, David P. Toczyski. We have developed a technique, called Ubiquitin Ligase Substrate Trapping, for the isolation of ubiquitinated substrates in complex with their ubiquitin ligase (E3). By fusing a ubiquitin-<....

Targeting MLL1 H3K4 Methyltransferase Activity in Mixed-Lineage Leukemia

Molecular Cell (most read) - Thu, 2014-01-02 02:00
Fang Cao, Elizabeth C. Townsend, Hacer Karatas, Jing Xu, Li Li, Shirley Lee, Liu Liu, Yong Chen, Peter Ouillette, Jidong Zhu, Jay L. Hess, Peter Atadja, Ming Lei, Zhaohui S. Qin, Sami Malek, Shaomeng Wang, Yali Dou. Here we report a comprehensive characterization of our recently developed inhibitor MM-401 that targets the MLL1 H3K4 methyltransferase activity. MM-401 is able to specifically inhibit MLL1 activi....

The Histone H3 Lysine 9 Methyltransferases G9a and GLP Regulate Polycomb Repressive Complex 2-Mediated Gene Silencing

Molecular Cell (most read) - Thu, 2014-01-02 02:00
Chiara Mozzetta, Julien Pontis, Lauriane Fritsch, Philippe Robin, Manuela Portoso, Caroline Proux, Raphaël Margueron, Slimane Ait-Si-Ali. G9a/GLP and Polycomb Repressive Complex 2 (PRC2) are two major epigenetic silencing machineries, which in particular methylate histone H3 on lysines 9 and 27 (H3K9 and H3K27), respectively. Althou....

An Effective Approach to Prevent Immune Rejection of Human ESC-Derived Allografts

Stem Cell: most read - Thu, 2014-01-02 02:00
Zhili Rong, Meiyan Wang, Zheng Hu, Martin Stradner, Shengyun Zhu, Huijuan Kong, Huanfa Yi, Ananda Goldrath, Yong-Guang Yang, Yang Xu, Xuemei Fu. Human embryonic stem cells (hESCs) hold great promise for cell therapy as a source of diverse differentiated cell types. One key bottleneck to realizing such potential is allogenic immune rejectio....
Categories: Biology, Journals, Stem Cells

Human Pluripotent Stem Cell Culture: Considerations for Maintenance, Expansion, and Therapeutics

Stem Cell: most read - Thu, 2014-01-02 02:00
Kevin G. Chen, Barbara S. Mallon, Ronald D.G. McKay, Pamela G. Robey. Human pluripotent stem cells (hPSCs) provide powerful resources for application in regenerative medicine and pharmaceutical development. In the past decade, various methods have been developed for....
Categories: Biology, Journals, Stem Cells

Highly Efficient Self-Replicating RNA Enzymes

Chemistry and Biology (most read) - Thu, 2014-01-02 02:00
Michael P. Robertson, Gerald F. Joyce. An RNA enzyme has been developed that catalyzes the joining of oligonucleotide substrates to form additional copies of itself, undergoing self-replication with exponential growth. The enzyme also ....
Categories: Biology, Chemistry, Journals

Synergy between Multiple Microtubule-Generating Pathways Confers Robustness to Centrosome-Driven Mitotic Spindle Formation

Developmental Cell (most read) - Thu, 2014-01-02 02:00
Daniel Hayward, Jeremy Metz, Claudia Pellacani, James G. Wakefield. The mitotic spindle is defined by its organized, bipolar mass of microtubules, which drive chromosome alignment and segregation. Although different cells have been shown to use different molecular....
Categories: Biology, Journals

Gamete Attachment Requires GEX2 for Successful Fertilization in Arabidopsis

Current Biology - Thu, 2014-01-02 02:00
Toshiyuki Mori, Tomoko Igawa, Gen Tamiya, Shin-ya Miyagishima, Frédéric Berger. Fertilization requires recognition, attachment, and membrane fusion between gametes. In metazoans, rapidly evolving surface proteins contribute to gamete recognition and adhesion [1]. Flowering pl....
Categories: Biology, Journals

A Novel Analgesic Isolated from a Traditional Chinese Medicine

Current Biology - Thu, 2014-01-02 02:00
Yan Zhang, Chaoran Wang, Lien Wang, Gregory Scott Parks, Xiuli Zhang, Zhimou Guo, Yanxiong Ke, Kang-Wu Li, Mi Kyeong Kim, Benjamin Vo, Emiliana Borrelli, Guangbo Ge, Ling Yang, Zhiwei Wang, M. Julia Garcia-Fuster, Z. David Luo, Xinmiao Liang, Olivier Civelli. BackgroundCurrent pain management is limited, in particular, with regard to chronic pain. In an attempt to discover novel analgesics, we combined the approach developed to characterize traditional....
Categories: Biology, Journals

Meiosis and Haploid Gametes in the Pathogen Trypanosoma brucei

Current Biology - Thu, 2014-01-02 02:00
Lori Peacock, Mick Bailey, Mark Carrington, Wendy Gibson. In eukaryote pathogens, sex is an important driving force in spreading genes for drug resistance, pathogenicity, and virulence [1]. For the parasitic trypanosomes that cause African sleeping sickn....
Categories: Biology, Journals

The Leucine-Rich Repeat Receptor Kinase BIR2 Is a Negative Regulator of BAK1 in Plant Immunity

Current Biology - Thu, 2014-01-02 02:00
Thierry Halter, Julia Imkampe, Sara Mazzotta, Michael Wierzba, Sandra Postel, Christoph Bücherl, Christian Kiefer, Mark Stahl, Delphine Chinchilla, Xiaofeng Wang, Thorsten Nürnberger, Cyril Zipfel, Steven Clouse, Jan Willem Borst, Sjef Boeren, Sacco C. de Vries, Frans Tax, Birgit Kemmerling. BackgroundTransmembrane leucine-rich repeat (LRR) receptors are commonly used innate immune receptors in plants and animals but can also sense endogenous signals to regulate development. BAK1 is a....
Categories: Biology, Journals

Coordination of Rho Family GTPase Activities to Orchestrate Cytoskeleton Responses during Cell Wound Repair

Current Biology - Thu, 2014-01-02 02:00
Maria Teresa Abreu-Blanco, Jeffrey M. Verboon, Susan M. Parkhurst. BackgroundCells heal disruptions in their plasma membrane using a sophisticated, efficient, and conserved response involving the formation of a membrane plug and assembly of an actomyosin ring. He....
Categories: Biology, Journals

Dynamic Facial Expressions of Emotion Transmit an Evolving Hierarchy of Signals over Time

Current Biology - Thu, 2014-01-02 02:00
Rachael E. Jack, Oliver G.B. Garrod, Philippe G. Schyns. Designed by biological [1, 2] and social [3] evolutionary pressures, facial expressions of emotion comprise specific facial movements [4–8] to support a near-optimal system of signaling and decodi....
Categories: Biology, Journals

Regulation of Transcriptional Bursting by a Naturally Oscillating Signal

Current Biology - Thu, 2014-01-02 02:00
Adam M. Corrigan, Jonathan R. Chubb. Transcription is highly stochastic, occurring in irregular bursts [1–3]. For temporal and spatial precision of gene expression, cells must somehow deal with this noisy behavior. To address how thi....
Categories: Biology, Journals

Regulation of transcription factor activity by interconnected post-translational modifications

Trends in Pharmacological Sciences - Mon, 2013-12-30 02:00
Theresa M. Filtz, Walter K. Vogel, Mark Leid.
• Transcription factors integrate extracellular signals.
• Transcription factor PTMs underlie the signaling mechanism to the nucleus.
• PTMs control every aspect ....
Categories: Biology, Healthcare, Journals

Identification of a Major Determinant for Serine-Threonine Kinase Phosphoacceptor Specificity

Molecular Cell (most read) - Thu, 2013-12-26 02:00
Catherine Chen, Byung Hak Ha, Anastasia F. Thévenin, Hua Jane Lou, Rong Zhang, Kevin Y. Yip, Jeffrey R. Peterson, Mark Gerstein, Philip M. Kim, Panagis Filippakopoulos, Stefan Knapp, Titus J. Boggon, Benjamin E. Turk. Eukaryotic protein kinases are generally classified as being either tyrosine or serine-threonine specific. Though not evident from inspection of their primary sequences, many serine-threonine kina....

Interactions between JARID2 and Noncoding RNAs Regulate PRC2 Recruitment to Chromatin

Molecular Cell (most read) - Thu, 2013-12-26 02:00
Syuzo Kaneko, Roberto Bonasio, Ricardo Saldaña-Meyer, Takahaki Yoshida, Jinsook Son, Koichiro Nishino, Akihiro Umezawa, Danny Reinberg. JARID2 is an accessory component of Polycomb repressive complex-2 (PRC2) required for the differentiation of embryonic stem cells (ESCs). A role for JARID2 in the recruitment of PRC2 to tar....

Polycomb Potentiates Meis2 Activation in Midbrain by Mediating Interaction of the Promoter with a Tissue-Specific Enhancer

Developmental Cell (most read) - Thu, 2013-12-26 02:00
Takashi Kondo, Kyoichi Isono, Kaori Kondo, Takaho A. Endo, Shigeyoshi Itohara, Miguel Vidal, Haruhiko Koseki. Polycomb-group (PcG) proteins mediate repression of developmental regulators in a reversible manner, contributing to their spatiotemporally regulated expression. However, it is poorl....
Categories: Biology, Journals