Rachel H. Roberts-Galbraith, Melanie D. Ohi, Bryan A. Ballif, Jun-Song Chen, Ian McLeod, W. Hayes McDonald, Steven P. Gygi, John R. Yates, Kathleen L. Gould. Cytokinesis in Schizosaccharomyces pombe requires the function of Cdc15, the founding member of the pombe cdc15 homology (PCH) family of proteins. As an early, abundant contractile r....

Dong Min Shin, Shmuel Muallem. High-Ca2+ microdomains at ER-mitochondrial junctions have been presumed to exist. Csordás et al. (2010) and Giacomello et al. (2010) report the Ca2+ dynamics at the outer mit....

György Csordás, Péter Várnai, Tünde Golenár, Swati Roy, George Purkins, Timothy G. Schneider, Tamás Balla, György Hajnóczky. The ER-mitochondrial junction provides a local calcium signaling domain that is critical for both matching energy production with demand and the control of apoptosis. Here, we visualize ER-mitocho....

Jason S. Lee, Yunho Kim, Ik Soo Kim, Bogyou Kim, Hee June Choi, Ji Min Lee, Hi-Jai R. Shin, Jung Hwa Kim, Ji-Young Kim, Sang-Beom Seo, Ho Lee, Olivier Binda, Or Gozani, Gregg L. Semenza, Minhyung Kim, Keun Il Kim, Daehee Hwang, Sung Hee Baek. Lysine methylation within histones is crucial for transcriptional regulation and thus links chromatin states to biological outcomes. Although recent studies have extended lysine methylation to non....

Haroula Kontaki, Iannis Talianidis. Histone-modifying enzymes can regulate DNA damage-induced apoptosis through modulation of p53 function. Here, we show that, in p53-deficient tumor cells, Set9 and LSD1 regulate DNA damage-induced ....

Raga Krishnakumar, W. Lee Kraus. The abundant nuclear enzyme PARP-1, a multifunctional regulator of chromatin structure, transcription, and genomic integrity, plays key roles in a wide variety of processes in the nucleus. Recent ....

Agata Smogorzewska, Rohini Desetty, Takamune T. Saito, Michael Schlabach, Francis P. Lach, Mathew E. Sowa, Alan B. Clark, Thomas A. Kunkel, J. Wade Harper, Monica P. Colaiácovo, Stephen J. Elledge. The Fanconi anemia (FA) pathway is responsible for interstrand crosslink repair. At the heart of this pathway is the FANCI-FAND2 (ID) complex, which, upon ubiquitination by the FA core complex, tr....

Yujing Zhang, Danqing Liu, Xi Chen, Jing Li, Limin Li, Zhen Bian, Fei Sun, Jiuwei Lu, Yuan Yin, Xing Cai, Qi Sun, Kehui Wang, Yi Ba, Qiang Wang, Dongjin Wang, Junwei Yang, Pingsheng Liu, Tao Xu, Qiao Yan, Junfeng Zhang, Ke Zen, Chen-Yu Zhang. MicroRNAs (miRNAs) are a class of noncoding RNAs that regulate target gene expression at the posttranscriptional level. Here, we report that secreted miRNAs can serve as signaling molecules mediat....

Adele Adamo, Spencer J. Collis, Carrie A. Adelman, Nicola Silva, Zuzana Horejsi, Jordan D. Ward, Enrique Martinez-Perez, Simon J. Boulton, Adriana La Volpe. Fanconi anemia (FA) is a complex cancer susceptibility disorder associated with DNA repair defects and infertility, yet the precise function of the FA proteins in genome maintenance remains unclea....

Alex S. Flynt, Jaclyn C. Greimann, Wei-Jen Chung, Christopher D. Lima, Eric C. Lai. microRNAs (miRNAs) are ∼22 nucleotide regulatory RNAs derived from hairpins generated either by Drosha cleavage (canonical substrates) or by splicing and debranching of short introns (mirtrons). T....

Hailing Jin, Jian-Kang Zhu. In this issue of Molecular Cell, Lee et al. show that Neuropsora crassa uses several Dicer-dependent and -independent pathways to generate miRNA-like RNAs and small-interfering RNAs.....

Joris J. Benschop, Nathalie Brabers, Dik van Leenen, Linda V. Bakker, Hanneke W.M. van Deutekom, Nynke L. van Berkum, Eva Apweiler, Philip Lijnzaad, Frank C.P. Holstege, Patrick Kemmeren. Analyses of biological processes would benefit from accurate definitions of protein complexes. High-throughput mass spectrometry data offer the possibility of systematically defining protein compl....

Ji-Hong Lim, Yoon-Mi Lee, Yang-Sook Chun, Junjie Chen, Ja-Eun Kim, Jong-Wan Park. To survive in hypoxic environments, organisms must be able to cope with redox imbalance and oxygen deficiency. The SIRT1 deacetylase and the HIF-1α transcription factor act as redox and oxy....

Chanseok Shin, Jin-Wu Nam, Kyle Kai-How Farh, H. Rosaria Chiang, Alena Shkumatava, David P. Bartel. Most metazoan microRNA (miRNA) target sites have perfect pairing to the seed region, located near the miRNA 5′ end. Although pairing to the 3′ region sometimes supplements seed matches or compensa....

Fedor V. Karginov, Sihem Cheloufi, Mark M.W. Chong, Alexander Stark, Andrew D. Smith, Gregory J. Hannon. The life span of a mammalian mRNA is determined, in part, by the binding of regulatory proteins and small RNA-guided complexes. The conserved endonuclease activity of Argonaute2 requires extensive....

Shintaro Iwasaki, Maki Kobayashi, Mayuko Yoda, Yuriko Sakaguchi, Susumu Katsuma, Tsutomu Suzuki, Yukihide Tomari. Small silencing RNAs—small interfering RNAs (siRNAs) or microRNAs (miRNAs)—direct posttranscriptional gene silencing of their mRNA targets as guides for the RNA-induced silencing complex (RISC). B....

Aditi Kanhere, Keijo Viiri, Carla C. Araújo, Jane Rasaiyaah, Russell D. Bouwman, Warren A. Whyte, C. Filipe Pereira, Emily Brookes, Kimberly Walker, George W. Bell, Ana Pombo, Amanda G. Fisher, Richard A. Young, Richard G. Jenner. Polycomb proteins maintain cell identity by repressing the expression of developmental regulators specific for other cell types. Polycomb repressive complex-2 (PRC2) catalyzes trimethylation of hi....

Jason M. Winget, Thibault Mayor. Ubiquitin is attached to a large number of proteins and gives rise to signaling events that modulate many cellular functions. These signals are often based on the recognition of polyubiquitin chai....

Galit Lahav. This article is for women who ask whether it is possible to combine motherhood with academia and still be successful and happy. It is also for those working with, bosses of, or married to such wom....

Uri Alon. Choosing good problems is essential for being a good scientist. But what is a good problem, and how do you choose one? The subject is not usually discussed explicitly within our profession. Scient....